This guidance is intended to provide recommendations for the collection and processing of ticks for tick and pathogen surveillance efforts. There are several existing guidance documents for tick surveillance (listed separately in references) and these may be consulted for additional information. Herein, we provide a concise guide for those who are starting tick surveillance activities or those who wish to standardize practices and methods in support of public health. There are three families of ticks (Acari: Ixodidae), with two families (Argasidae and Ixodidae) in the United States. General guidance on the surveillance of soft ticks (Argasidae) is available from Manzano-Roman et al. (2012). CDC tick surveillance guidance was issued for prostriate hard ticks (Ixodes spp.) in September 2018 (revised in April 2019; see www.cdc.gov/ticks/surveillance/). The guidance herein serves to expand surveillance guidance to the metastriate or non-Ixodes hard tick genera (Acari: Ixodidae). This diverse group of ticks may be found in a wider variety of habitats and environmental conditions. This guidance defines the information desired from tick surveillance including where these ticks occur, when the different stages are most active during the year, and which human pathogens are of greatest local concern.