This manual contains comprehensive information on epidemiologic reporting between jurisdictions, investigation protocols, case definitions, surveillance data, security and legal elements of an epidemiologic investigation, training for newer epidemiologists, sample templates for standing orders, and sample press releases. Job/role descriptions at all levels of an epidemiologic investigation clearly delineate responsibilities during a response. Much of the information in the manual is informed by site visits to collect best practices and practices in use for epidemiologic investigation. This tool was identified through NACCHOs Resource Portfolio Project on the CDC Public Health Preparedness Capabilities. Through this project, NACCHO worked with the Center for Infectious Disease Research and Policy (CIDRAP) to identify, research, and validate tools, practices, and products that can assist LHDs to address challenges in meeting select PHEP capabilities.