NACCHO's Organizational Culture of Quality Self-Assessment Tool (SAT) enables beginner, intermediate, and advanced local health departments (LHDs) to conduct a baseline assessment of organizational maturity within critical aspects of a culture of quality, revealing opportunities for improvement, and informing a trajectory for next steps to reaching a culture of quality. The tool is intentionally designed to complement NACCHO's Roadmap to a Culture of Quality which provides high level guidance on progressing through six phases of QI maturity by incrementally building six foundational elements of a quality culture. The SAT is framed around these six foundational elements and further sub-divided into 20 sub-elements, providing an in-depth assessment of the people, systems, and structures that support a quality culture. Based on assessment results, this tool also identifies tangible transition strategies for moving along the Roadmap. The SAT contains three main components: 1) Diagnostic Statements to assess the current organizational culture and identify priority gaps; 2) Corresponding Transition Strategies that provide suggested actions to close priority gaps; and 3) A Scoring Summary sheet to document results and assist with organizational planning efforts.